Heparin modulates human intestinal smooth muscle cell proliferation, protein synthesis, and lattice contraction
| Autor: | Hilary A. Perr, H. Paul Ehrlich, Robert F. Diegelmann, David E.M. Drucker, Martin F. Graham |
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| Rok vydání: | 1987 |
| Předmět: |
Photomicrography
Contraction (grammar) Cell division Cell Extracellular matrix In vivo medicine Humans Cells Cultured Cytoskeleton Dose-Response Relationship Drug Hepatology Heparin Cell growth Chemistry Gastroenterology Muscle Smooth In vitro Culture Media Cell biology Jejunum medicine.anatomical_structure Biochemistry Protein Biosynthesis Collagen Cell Division medicine.drug |
| Zdroj: | Gastroenterology. 93:801-809 |
| ISSN: | 0016-5085 |
| Popis: | The effect of heparin on human intestinal smooth muscle cell proliferation, collagen and noncollagen protein synthesis, and collagen lattice contraction was studied in vitro. Proliferation of serum-stimulated cells was inhibited in a concentration-related fashion by continuous exposure to heparin. The inhibition of proliferation was reversible when heparin was removed from the culture medium. Collagen synthesis was inhibited by 24-h exposure to heparin, but only during that phase of culture (8-12 days) when collagen synthesis was maximal. Noncollagen protein synthesis was down-regulated by 24-h exposure to heparin at all phases of culture tested (5-21 days). Heparin also abolished the contraction of collagen lattices populated by human intestinal smooth muscle cells. These studies demonstrate that heparin plays a significant role in the modulation of human intestinal smooth muscle cell behavior in vitro and suggest that a similar role is played by heparinlike components of the extracellular matrix in vivo. |
| Databáze: | OpenAIRE |
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