Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues
| Autor: | Hitoshi Kawase, Makoto Hiraga, Yuichi Ishihara, Shin Sugiishi, Masamitsu Ito, Akio Mitani, Norimitsu Shirozu, Nobuo Yoshinari, Toshihide Noguchi, Kyoko Arakawa, Masanori Matsuoka, Boku Bito |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty medicine.drug_class Gingiva Inflammation Scaling and root planing Medicine Humans RNA Messenger Receptor Periodontitis In Situ Hybridization business.industry Interleukin Receptors Interleukin-1 Gingival Crevicular Fluid Middle Aged medicine.disease Receptor antagonist Chronic periodontitis Immunohistochemistry Interleukin 1 receptor antagonist Chronic Disease Periodontics Dental Scaling Female medicine.symptom business Interleukin-1 |
| Zdroj: | Journal of periodontal research. 39(3) |
| ISSN: | 0022-3484 |
| Popis: | Objective: The purpose of this study was to evaluate the relationship between the clinical changes after non-surgical periodontal therapy and interleukin 1 (IL-1) in gingival crevicular fluid (GCF) and gingival tissues from patients with chronic periodontitis. Background: The inflammatory responses mediated by IL-1 play an important role in periodontal tissue destruction. Although numerous studies have attempted to elucidate the dynamic movement involved in chronic periodontitis, the results have often conflicted. Such discrepancies may have been due to the inability to determine clinical disease activity. Methods: Seven patients with chronic periodontitis were examined. The severity of periodontal inflammation was expressed using clinical parameters before and after a scaling and root planing (SRP) procedure. The amounts and concentrations of IL-1α, IL-1β and IL-1 receptor antagonist in GCF were measured by enzyme-linked immunosorbent assay (ELISA) and IL-1 activity index was calculated. A needle biopsy in matching gingival tissues was also performed before and after the SRP procedure. The localization and mRNA expression of IL-1β were determined using histological methods. Results: Clinical parameters improved slightly after the SRP procedure. Only the probing pocket depth (PPD) was reduced significantly (p |
| Databáze: | OpenAIRE |
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