Probing the binding specificities of human Siglecs by cell-based glycan arrays
| Autor: | James C. Paulson, Julie Van Coillie, Henrik Clausen, Zhang Yang, Sam J. Moons, Ryan McBride, Gosse J. Adema, Corwin M. Nycholat, Sanae Furukawa, Thomas J. Boltje, Ronald L. Schnaar, Rebecca Nason, Daniel Madriz Sørensen, Lingbo Sun, Steven Arbitman, Steve M. Fernandes, Yoshiki Narimatsu, Christian Büll |
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| Rok vydání: | 2021 |
| Předmět: |
Glycan
Sialyltransferase Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] Context (language use) Synthetic Organic Chemistry Interactome Epitope 03 medical and health sciences chemistry.chemical_compound Gene Knockout Techniques 0302 clinical medicine Polysaccharides Humans 030304 developmental biology Sialic Acid Binding Immunoglobulin-like Lectins 0303 health sciences Multidisciplinary biology Chemistry Mucin-1 SIGLEC Biological Sciences respiratory system Sialyltransferases Cell biology Sialic acid carbohydrates (lipids) HEK293 Cells Tissue Array Analysis 030220 oncology & carcinogenesis Sialome biology.protein |
| Zdroj: | Proceedings of the National Academy of Sciences USA, 118, 17 Proceedings of the National Academy of Sciences Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences USA, 118 |
| ISSN: | 0027-8424 |
| Popis: | Item does not contain fulltext Siglecs are a family of sialic acid-binding receptors expressed by cells of the immune system and a few other cell types capable of modulating immune cell functions upon recognition of sialoglycan ligands. While human Siglecs primarily bind to sialic acid residues on diverse types of glycoproteins and glycolipids that constitute the sialome, their fine binding specificities for elaborated complex glycan structures and the contribution of the glycoconjugate and protein context for recognition of sialoglycans at the cell surface are not fully elucidated. Here, we generated a library of isogenic human HEK293 cells with combinatorial loss/gain of individual sialyltransferase genes and the introduction of sulfotransferases for display of the human sialome and to dissect Siglec interactions in the natural context of glycoconjugates at the cell surface. We found that Siglec-4/7/15 all have distinct binding preferences for sialylated GalNAc-type O-glycans but exhibit selectivity for patterns of O-glycans as presented on distinct protein sequences. We discovered that the sulfotransferase CHST1 drives sialoglycan binding of Siglec-3/8/7/15 and that sulfation can impact the preferences for binding to O-glycan patterns. In particular, the branched Neu5Acα2-3(6-O-sulfo)Galβ1-4GlcNAc (6'-Su-SLacNAc) epitope was discovered as the binding epitope for Siglec-3 (CD33) implicated in late-onset Alzheimer's disease. The cell-based display of the human sialome provides a versatile discovery platform that enables dissection of the genetic and biosynthetic basis for the Siglec glycan interactome and other sialic acid-binding proteins. |
| Databáze: | OpenAIRE |
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