Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
| Autor: | John E. Hall, Jonathan P. Hosler, Michael E. Hall, Romain Harmancey, Jessica M. Wiseman, Sadia Ashraf, Kristin S. Edwards, F. N. Gava, Tyler M. Lomax |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Physiology Ischemia Respiratory chain Myocardial Reperfusion Injury 030204 cardiovascular system & hematology Mitochondrion Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences Gene Knockout Techniques Mice 0302 clinical medicine Insulin resistance Physiology (medical) Internal medicine Uncoupling protein medicine Animals Uncoupling Protein 3 Myocardial infarction Beta oxidation UCP3 Chemistry Myocardium Fatty Acids Type 2 diabetes Original Contribution medicine.disease 3. Good health Rats 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 Mitochondrial function Cardiology and Cardiovascular Medicine Oxidation-Reduction Cardiac metabolism |
| Zdroj: | Basic Research in Cardiology |
| ISSN: | 1435-1803 |
| Popis: | Patients with insulin resistance and type 2 diabetes have poor cardiac outcomes following myocardial infarction (MI). The mitochondrial uncoupling protein 3 (UCP3) is down-regulated in the heart with insulin resistance. We hypothesized that decreased UCP3 levels contribute to poor cardiac recovery following ischemia/reperfusion (I/R). After confirming that myocardial UCP3 levels were systematically decreased by 20–49% in animal models of insulin resistance and type 2 diabetes, we genetically engineered Sprague–Dawley rats with partial loss of UCP3 (ucp3+/−). Wild-type littermates (ucp3+/+) were used as controls. Isolated working hearts from ucp3+/− rats were characterized by impaired recovery of cardiac power and decreased long-chain fatty acid (LCFA) oxidation following I/R. Mitochondria isolated from ucp3+/− hearts subjected to I/R in vivo displayed increased reactive oxygen species (ROS) generation and decreased respiratory complex I activity. Supplying ucp3+/− cardiac mitochondria with the medium-chain fatty acid (MCFA) octanoate slowed electron transport through the respiratory chain and reduced ROS generation. This was accompanied by improvement of cardiac LCFA oxidation and recovery of contractile function post ischemia. In conclusion, we demonstrated that normal cardiac UCP3 levels are essential to recovery of LCFA oxidation, mitochondrial respiratory capacity, and contractile function following I/R. These results reveal a potential mechanism for the poor prognosis of type 2 diabetic patients following MI and expose MCFA supplementation as a feasible metabolic intervention to improve recovery of these patients at reperfusion. Electronic supplementary material The online version of this article (10.1007/s00395-018-0707-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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