Regulation of Human Insulin Receptor RNA Splicing in HepG2 Cells: Effects of Glucocorticoid and Low Glucose Concentration
| Autor: | Holger Luthman, Luo-Sheng Li, S. Norgren |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Carcinoma Hepatocellular medicine.medical_treatment Biophysics In Vitro Techniques Polymerase Chain Reaction Biochemistry Dexamethasone Exon Insulin receptor substrate Internal medicine Tumor Cells Cultured medicine Humans Insulin Glucose homeostasis RNA Messenger Insulin-Like Growth Factor I Molecular Biology biology Alternative splicing RNA Cell Biology Receptor Insulin Alternative Splicing Insulin receptor Glucose Endocrinology Gene Expression Regulation RNA splicing biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 199:277-284 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.1225 |
| Popis: | Human insulin receptor RNA is expressed in two alternatively spliced forms (Ex. 11- and Ex. 11+) encoding protein isoforms with discrete functional differences. In vivo, the splicing varies between tissues and changes concomitantly with the control of glucose homeostasis. Recently, dexamethasone has been described to increase the relative expression of Ex. 11+ RNA molecules. In this study, we confirm that dexamethasone dose-dependently increases inclusion of exon 11, and demonstrate that low concentrations of glucose decrease inclusion. No effect was observed of either insulin or IGF1. These results suggest that hormonal as well as metabolic factors regulate the splicing of insulin receptor RNA in HepG2 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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