Efficacy of netropsin dihydrochloride against the viability, cytopathogenicity and hemolytic activity of Trichomonas vaginalis clinical isolates
| Autor: | Maha M M Abou Gamra, Fatima Zahran, Hayam Mohamed Ezz Eldin, Hanan Hussein Kamel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.drug_class 030106 microbiology Antibiotics Drug Resistance Virulence Drug resistance medicine.disease_cause Hemolysis Madin Darby Canine Kidney Cells Microbiology 03 medical and health sciences chemistry.chemical_compound Dogs 0302 clinical medicine Parasitic Sensitivity Tests Metronidazole Trichomonas vaginalis medicine Animals Humans Pharmacology (medical) Trophozoites 030212 general & internal medicine Netropsin Haemolysis medicine.disease Anti-Bacterial Agents Infectious Diseases chemistry Vagina Female Trichomonas Vaginitis medicine.drug |
| Zdroj: | Journal of Infection and Chemotherapy. 25:955-964 |
| ISSN: | 1341-321X |
| DOI: | 10.1016/j.jiac.2019.05.015 |
| Popis: | Trichomonas vaginalis (T. vaginalis) is a common sexually transmitted infection, affecting the urogenital tract. Trichomoniasis is customarily treated with metronidazole (MTZ). MTZ is known to cause undesirable side effects and there is several reports on MTZ resistant T. vaginalis. Thus, the present study aimed to in-vitro evaluate the activity of DNA minor groove binder drug ''Netropsin dihydrochloride'' against metronidazole-sensitive T. vaginalis isolates (G and U isolates) and resistant T. vaginalis isolate (ATCC50138) (R isolate). Netropsin was tested at concentrations ranging from 3.5 to 200 μg/ml. It showed effectiveness against all isolates with MLC of 12.5 μg/ml for G and U isolates and of 25 μg/ml for R isolate. Cytotoxicity assay of isolates exposed to the respective MLC of netropsin for 42 h showed a highly significant reduction in the death percentage of MCDK cell line as compared to the effect elicited by drug free controls. The hemolytic activity was evaluated by hemolytic assay and by monitoring the interaction of T. vaginalis isolates with human erythrocytes by inverted microscopy and scanning electron microscopy. The hemolytic assay showed (0%) hemolysis of RBCs incubated with T. vaginalis isolates treated with the corresponding MLC of netropsin for 24 h. Scanning electron microscopy revealed cytoskeletal deformities of netropsin treated isolates. Taken together, these observations suggest that netropsin is a promising therapy for T. vaginalis infection affecting its viability, virulence, cytopathogenic and hemolytic activity with a mechanism of action that might overcome T. vaginalis resistance to metronidazole. |
| Databáze: | OpenAIRE |
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