Pivotal Role of Lesional and Perilesional T/B Lymphocytes in Pemphigus Pathogenesis
| Autor: | Weihong Zeng, Haiqin Zhu, Huijie Yuan, Renchao Xu, Zhicui Liu, Jie Zheng, Ying Wang, Weiting Cong, Xiaochun Fei, Meng Pan, Shengru Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Enzyme-Linked Immunospot Assay Pathology medicine.medical_specialty T-Lymphocytes Blotting Western Enzyme-Linked Immunosorbent Assay Dermatology Biology Biochemistry 03 medical and health sciences Immune system Cell Movement Reference Values medicine Humans Molecular Biology B-Lymphocytes integumentary system Desmoglein 1 Interleukins ELISPOT Biopsy Needle Interleukin-17 Pemphigus vulgaris CCL19 Role Cell Biology medicine.disease Immunohistochemistry Pemphigus 030104 developmental biology Case-Control Studies Immunology biology.protein Female Bullous pemphigoid Interleukin 17 Antibody |
| Zdroj: | Journal of Investigative Dermatology. 137:2362-2370 |
| ISSN: | 0022-202X |
| DOI: | 10.1016/j.jid.2017.05.032 |
| Popis: | Pemphigus is a skin and mucosal membrane-targeting autoimmune bullous disease. Previous studies have shown that circulating anti-desmoglein1/3 antibodies are pathogenic and mediate blister formation. However, the role of infiltrating immune cells in lesional skin has not been fully investigated. In this study we showed that there existed a large number of B and T lymphocytes and plasma cells in the skin lesions by immunohistochemistry and immunofluorescence staining. In addition, a significantly increased number of Dsg1- and Dsg3-specific B cells could be identified by flow cytometric analysis or enzyme-linked immunospot technique (i.e., ELISPOT) assay. Furthermore, anti-Dsg1 and Dsg3 antibodies could be detected from the supernatant of in vitro cultures with isolated lymphocytes from lesional skin. We found that most T lymphocytes infiltrating pemphigus vulgaris lesions were CD4+ T helper cells expressing IL-21 and IL-17a but not typical T follicular helper cells expressing CXCR5. Additionally, our microarray assay showed that the level of chemokine CCL19 was significantly elevated, suggesting active T-/B-lymphocyte trafficking and aggregation in the pemphigus vulgaris lesions. Collectively, our results suggest a critical role of locally infiltrating lymphocytes in pemphigus pathogenesis. |
| Databáze: | OpenAIRE |
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