CD14-Negative Isolation Enhances Chondrogenesis in Synovial Fibroblasts
| Autor: | Deborah McK. Ciombor, Roy K. Aaron, Yuexin Ren, Ming Pei, Bahar Bilgen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Swine
Population Cell Culture Techniques Biomedical Engineering Bioengineering Biochemistry Transforming Growth Factor beta1 Biomaterials Extracellular matrix medicine Animals education Cells Cultured education.field_of_study Tissue Engineering biology Cartilage Synovial Membrane CD44 Mesenchymal stem cell Original Articles Fibroblasts Chondrogenesis Molecular biology Hyaluronan Receptors medicine.anatomical_structure Synovial Cell Immunology biology.protein Synovial membrane |
| Zdroj: | Tissue Engineering Part A. 15:3261-3270 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2008.0273 |
| Popis: | Synovial membrane has been shown to contain mesenchymal stem cells. We hypothesized that an enriched population of synovial fibroblasts would undergo chondrogenic differentiation and secrete cartilage extracellular matrix to a greater extent than would a mixed synovial cell population (MSCP). The optimum doses of transforming growth factor beta 1 (TGF-beta1) and insulin-like growth factor 1 (IGF-1) for chondrogenesis were investigated. CD14-negative isolation was used to obtain a porcine cell population enriched in type-B synovial fibroblasts (SFB) from an MSCP. The positive cell surface markers in SFB were CD90, CD44, and cadherin-11. SFB and MSCP were cultured in the presence of 20 ng/mL TGF-beta1 for 7 days, and SFB were demonstrated to have higher chondrogenic potential. Further dose-response studies were carried out using the SFB cells and several doses of TGF-beta1 (2, 10, 20, and 40 ng/mL) and/or IGF-1 (1, 10, 100, and 500 ng/mL) for 14 days. TGF-beta1 supplementation was essential for chondrogenesis and prevention of cell death, whereas IGF-1 did not have a significant effect on the SFB cell number or glycosaminoglycan production. This study demonstrates that the CD14-negative isolation yields an enhanced cell population SFB that is more potent than MSCP as a cell source for cartilage tissue engineering. |
| Databáze: | OpenAIRE |
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