Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives
| Autor: | Polydefkis Hatzopoulos, Elisavet Angeli, Prokopios Magiatis, Margarita Thomopoulou, Konstantinos Koudounas, Aimilia Rigakou, Eleni Melliou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Upstream and downstream (transduction)
secoiridoids virus-induced gene silencing Plant Science olive SB1-1110 chemistry.chemical_compound Biosynthesis Oleuropein Gene silencing Olea europaea Original Research chemistry.chemical_classification biology Chemistry fungi one-dimensional quantitative nuclear magnetic resonance (1D-qNMR) food and beverages Plant culture biology.organism_classification Olive trees Enzyme Biochemistry Olea β-glucosidase oleuropein tobacco rattle virus Flux (metabolism) |
| Zdroj: | Frontiers in Plant Science, Vol 12 (2021) Frontiers in Plant Science |
| Popis: | Specialized metabolism is an evolutionary answer that fortifies plants against a wide spectrum of (a) biotic challenges. A plethora of diversified compounds can be found in the plant kingdom and often constitute the basis of human pharmacopeia. Olive trees (Olea europaea) produce an unusual type of secoiridoids known as oleosides with promising pharmaceutical activities. Here, we transiently silenced oleuropein β-glucosidase (OeGLU), an enzyme engaged in the biosynthetic pathway of secoiridoids in the olive trees. Reduction of OeGLU transcripts resulted in the absence of both upstream and downstream secoiridoids in planta, revealing a regulatory loop mechanism that bypasses the flux of precursor compounds toward the branch of secoiridoid biosynthesis. Our findings highlight that OeGLU could serve as a molecular target to regulate the bioactive secoiridoids in olive oils. |
| Databáze: | OpenAIRE |
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