Improvement of Monoclonal Antibody Production in Hybridoma Cells by Dimethyl Sulfoxide
| Autor: | Liang Deng, Marcio Voloch, Collette Cutler, Susan Cannon-Carlson, Yan Wang, Joseph Lepore, Wai Lam W. Ling |
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| Rok vydání: | 2003 |
| Předmět: |
Quality Control
Hybridomas Glycosylation Dose-Response Relationship Drug biology Cell Survival Dimethyl sulfoxide medicine.drug_class Antibodies Monoclonal Cell Count Monoclonal antibody Molecular biology Culture Media Dose–response relationship Titer chemistry.chemical_compound chemistry Bioreactor biology.protein medicine Dimethyl Sulfoxide Antibody Clone (B-cell biology) Biotechnology |
| Zdroj: | Biotechnology Progress. 19:158-162 |
| ISSN: | 8756-7938 |
| DOI: | 10.1021/bp020068d |
| Popis: | Hybridoma cultures are routinely used as a source for monoclonal antibody (mAb) production necessary for preclinical evaluation. However, these cultures typically have low volumetric and specific productivities. In this article, we examined the use and the timing of addition of dimethyl sulfoxide (DMSO) as a medium additive to improve mAb production in our hybridoma clone 19 (c19) cultures. From shake flask studies, we defined the optimal DMSO concentration and time of addition for improved productivity. This timing coordinated with high cell viability and density. Hybridoma cultures treated with DMSO up to 0.3% (v/v) possessed cell densities and viabilities comparable to untreated control. We demonstrated that 0.2% (v/v) DMSO added to shake flask cultures at their maximal viable cell densities resulted in a 2-fold increase in specific mAb production. This procedure was scaleable up to 20 L Cellbags (Wave Bioreactors) with similar titer improvement. Moreover, DMSO treatment did not affect the bioactivity or glycosylation profiles of the mAb. |
| Databáze: | OpenAIRE |
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