Apolipoprotein B48 metabolism in chylomicrons and very low‐density lipoproteins and its role in triglyceride transport in normo‐ and hypertriglyceridemic human subjects

Autor: Antti Hakkarainen, Linda Andersson, Carina Sihlbom, Sanni Söderlund, Martin Adiels, Juhani Kahri, Haihong Zhou, Jan Borén, Jesper Lundbom, Chris J. Packard, Elias Björnson, Niina Matikainen, Nina Lundbom, Marja-Riitta Taskinen, Annika Thorsell
Přispěvatelé: HUS Abdominal Center, Staff Services, CAMM - Research Program for Clinical and Molecular Metabolism, Research Programs Unit, University of Helsinki, Endokrinologian yksikkö, Clinicum, Department of Medicine, HUS Internal Medicine and Rehabilitation, Marja-Riitta Taskinen Research Group, HUS Medical Imaging Center, Department of Diagnostics and Therapeutics
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Very low-density lipoprotein
Apolipoprotein B
Lipoproteins
VLDL
030204 cardiovascular system & hematology
apoB100
B-48 TRANSPORT
chemistry.chemical_compound
0302 clinical medicine
apoB48
OF-FUNCTION MUTATIONS
Chylomicrons
RISK
Hypertriglyceridemia
education.field_of_study
PLASMA
biology
digestive
oral
and skin physiology
STABLE-ISOTOPE
Middle Aged
REMNANT CHOLESTEROL
3. Good health
Protein Transport
CARDIOVASCULAR-DISEASE
Apolipoprotein B-100
lipids (amino acids
peptides
and proteins)
medicine.medical_specialty
C-III
Lipolysis
Lipoproteins
Dietary lipid
Population
postpranidal lipid metabolism
stable isotopes
03 medical and health sciences
Internal medicine
Internal Medicine
medicine
Humans
education
Triglycerides
Triglyceride
Triglyceride transport
business.industry
medicine.disease
030104 developmental biology
Endocrinology
chemistry
kinetics
FAT
Heart Disease Risk Factors
3121 General medicine
internal medicine and other clinical medicine
RICH LIPOPROTEINS
biology.protein
Apolipoprotein B-48
business
Chylomicron
ISSN: 0954-6820
Popis: Background: \ud Renewed interest in triglyceride-rich lipoproteins as causative agents in cardiovascular disease mandates further exploration of the integrated metabolism of chylomicrons and very low-density lipoproteins (VLDL).\ud \ud Methods: \ud Novel tracer techniques and an integrated multi-compartmental model were used to determine the kinetics of apoB48- and apoB100-containing particles in the chylomicron and VLDL density intervals in 15 subjects with a wide range of plasma triglyceride levels.\ud \ud Results: \ud Following a fat-rich meal, apoB48 appeared in the chylomicron, VLDL1 and VLDL2 fractions in all subjects. Chylomicrons cleared rapidly from the circulation but apoB48-containing VLDL accumulated, and over the day were 3-fold higher in those with high versus low plasma triglyceride. ApoB48-containing particles were secreted directly into both the chylomicron and VLDL fractions at rates that were similar across the plasma triglyceride range studied. During fat absorption, whilst most triglyceride entered the circulation in chylomicrons, the majority of apoB48 particles were secreted into the VLDL density range.\ud \ud Conclusion: \ud The intestine secretes apoB48-containing particles not only as chylomicrons but also directly into the VLDL1 and VLDL2 density ranges both in the basal state and during dietary lipid absorption. Over the day, apoB48-containing particles appear to comprise about 20–25% of circulating VLDL and, especially in those with elevated triglycerides, form part of a slowly cleared ‘remnant’ particle population, thereby potentially increasing CHD risk. These findings provide a metabolic understanding of the potential consequences for increased CHD risk when slowed lipolysis leads to the accumulation of remnants, especially in individuals with hypertriglyceridemia.
Databáze: OpenAIRE
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