MicroRNAs: An adaptive mechanism in the pancreatic β-cell…and beyond?
| Autor: | Matthew N. Poy |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Endocrinology Diabetes and Metabolism Cell Computational biology Biology Bioinformatics 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin-Secreting Cells microRNA medicine Animals Humans Mechanism (biology) RNA Cell Differentiation Non-coding RNA Adaptation Physiological MicroRNAs 030104 developmental biology medicine.anatomical_structure Stem cell 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Best Practice & Research Clinical Endocrinology & Metabolism. 30:621-628 |
| ISSN: | 1521-690X |
| Popis: | Recent protocols have been developed to differentiate human stem cells and fibroblasts into insulin-producing cells capable of releasing the hormone in a glucose-stimulated manner. Limitations remain which prevent bringing these protocols to a clinical setting as these models must still undergo complete characterization. Advances in sequencing technologies have driven the identification of several non-coding RNA species including microRNAs (miRNAs). While their diversity and unique expression patterns across different tissues have made deciphering their precise functional role a significant challenge, studies using both cell lines and transgenic mouse models have made substantial progress in understanding their regulatory role on exocytosis and proliferation of the β-cell. These results also indicate miRNAs play an integral role in the fundamental mechanics of how the cell manages the balance between these independent functions. Continued investigation into miRNA function may uncover mechanisms which can be exploited to improve differentiation protocols in producing fully mature β-cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect