Extracellular neuraminidase production by group B streptococci
| Autor: | T W Milligan, D C Straus, S J Mattingly |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Ovalbumin
Immunology Serum albumin Neuraminidase Microbiology Streptococcus agalactiae Substrate Specificity Extracellular medicine Bovine serum albumin Serum Albumin biology Cell Cycle Mucin Proteolytic enzymes Human serum albumin Culture Media Chemically defined medium Infectious Diseases Biochemistry biology.protein Parasitology Peptide Hydrolases Research Article medicine.drug |
| Zdroj: | Infection and Immunity. 18:189-195 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.18.1.189-195.1977 |
| Popis: | Neuraminidase (sialidase) activity in concentrated culture filtrates of group B streptococci was measured with bovine submaxillary mucin as substrate. Group B streptococcal neuraminidase was not active on human alpha-1 acid glycoprotein and did not show increased activity on bovine submaxillary mucin that had been O-deacetylated by alkaline treatment. The enzyme was produced in a variety of media, including a chemically defined medium (FMC; Terleckyj et al., Infect. Immun. 11:649-655, 1975) supplemented with bovine serum albumin or human serum albumin. Maximal levels of activity were present in filtrates from cells grown in a dialyzable fraction of Todd-Hewitt broth harvested during the late exponential phase of growth. Dramatic decreases were seen when filtrates from the late stationary phase were assayed. The decrease in specific activity during the stationary phase was shown to be due to proteolytic digestion of neuraminidase and not to the elaboration of an extracellular neuraminic acid aldolase. |
| Databáze: | OpenAIRE |
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