DosiKit, a New Portable Immunoassay for Fast External Irradiation Biodosimetry
| Autor: | Sylvie Chevillard, Sandrine Altmeyer, Caroline Bettencourt, Marco Valente, X. Michel, Francis Herodin, François Desangles, F. Entine, Nicolas Ugolin, Jérôme Pateux, Guillaume Cosler, Flora Jourquin, Yannick Lecompte, Michel Drouet, Patrick Martigne, Julie Bensimon Etzol, Samuel Bouvet |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Time Factors Calibration curve Biophysics 030218 nuclear medicine & medical imaging 03 medical and health sciences Dicentric chromosome 0302 clinical medicine Biodosimetry medicine Humans Radiology Nuclear Medicine and imaging Irradiation Radiometry Immunoassay Radiation medicine.diagnostic_test business.industry Chemistry External irradiation Gold standard (test) Middle Aged Kinetics Blood 030220 oncology & carcinogenesis Calibration Female Dose rate Nuclear medicine business |
| Zdroj: | Radiation Research. 190:176 |
| ISSN: | 0033-7587 |
| Popis: | DosiKit is a new field-radiation biodosimetry immunoassay for rapid triage of individuals exposed to external total-body irradiation. Here, we report on the validation of this immunoassay in human blood cell extracts 0.5 h after in vitro exposure to 137Cs gamma rays, using γ-H2AX analysis. First, calibration curves were established for five donors at doses ranging from 0 to 10 Gy and dose rates ranging from ∼0.8 to ∼3 Gy/min. The calibration curves, together with a γ-H2AX peptide scale, enabled the definition of inter-experimental correction factors. Using previously calculated correction factors, blind dose estimations were performed at 0.5 h postirradiation, and DosiKit performance was compared against concomitant dicentric chromosome assay (DCA), the current gold standard for external irradiation biodosimetry. A prototype was then assembled and field tested. We show that, despite significant inter-individual variations, DosiKit can estimate total-body irradiation doses from 0.5 to 10 Gy with a strong l... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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