Induction of sulfadiazine resistance in vitro in Toxoplasma gondii
| Autor: | Isabelle Villena, Aline Schmid, Christelle Doliwa, Dominique Aubert, Frédéric Velard, Régine Geers, Sandie Escotte-Binet |
|---|---|
| Přispěvatelé: | Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Centre de Ressources Biologiques Toxoplasmes, Centre National de référence de la Toxoplasmose (CNRT) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
[SDV]Life Sciences [q-bio]
030231 tropical medicine Immunology Antiprotozoal Agents Drug Resistance Sulfadiazine Enzyme-Linked Immunosorbent Assay Drug resistance Microbiology Inhibitory Concentration 50 03 medical and health sciences 0302 clinical medicine Parasitic Sensitivity Tests Chlorocebus aethiops medicine Animals Inhibitory concentration 50 Vero Cells ComputingMilieux_MISCELLANEOUS 0303 health sciences biology 030306 microbiology Toxoplasma gondii General Medicine biology.organism_classification In vitro 3. Good health Infectious Diseases Vero cell Parasitology Toxoplasma medicine.drug |
| Zdroj: | Experimental Parasitology Experimental Parasitology, Elsevier, 2013, 133 (2), pp.131-136 |
| ISSN: | 0014-4894 1090-2449 |
| Popis: | We induced sulfadiazine resistance in two sulfadiazine sensitive strains of Toxoplasma gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC(50) values of sulfadiazine were 77 μg/mL for RH, 51 μg/mL for ME-49 and higher than 1000 μg/mL for the two natural resistant strains. Secondly, induced resistance of the strains by gradually increase sulfadiazine concentration was verified by this test, which resulted IC(50) values at higher than 1000 μg/mL. In conclusion we developed in vitro two sulfadiazine resistant strains called RH-R(SDZ) and ME-49-R(SDZ). These strains resistant to sulfadiazine would be useful to characterize resistance mechanisms to sulfadiazine. |
| Databáze: | OpenAIRE |
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