Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates
| Autor: | Adeeti Ullal, Cesar M. Castro, Vanessa M. Peterson, Ralph Weissleder, Dejan Juric, Sarit S. Agasti, Suan Tuang |
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| Rok vydání: | 2014 |
| Předmět: |
Biopsy
Fine-Needle Protein Array Analysis Biology Bioinformatics Antibodies Article Phosphatidylinositol 3-Kinases Single-cell analysis Cell Line Tumor Neoplasms Drug response Humans In patient Protein Kinase Inhibitors Phosphoinositide-3 Kinase Inhibitors Protein level Reproducibility of Results General Medicine Biopsy fine needle DNA Neoplasm Proteins Cancer cell Single-Cell Analysis Core biopsy Signal Transduction |
| Zdroj: | Science translational medicine. 6(219) |
| ISSN: | 1946-6242 |
| Popis: | Immunohistochemistry-based clinical diagnoses require invasive core biopsies and use a limited number of protein stains to identify and classify cancers. Here, we introduce a technology that allows analysis of hundreds of proteins from minimally invasive fine needle aspirates (FNA), which contain much smaller numbers of cells than core biopsies. The method capitalizes on DNA-barcoded antibody sensing where barcodes can be photo-cleaved and digitally detected without any amplification steps. Following extensive benchmarking in cell lines, this method showed high reproducibility and achieved single cell sensitivity. We used this approach to profile ~90 proteins in cells from FNAs and subsequently map patient heterogeneity at the protein level. Additionally, we demonstrate how the method could be used as a clinical tool to identify pathway responses to molecularly targeted drugs and to predict drug response in patient samples. This technique combines specificity with ease of use to offer a new tool for understanding human cancers and designing future clinical trials. |
| Databáze: | OpenAIRE |
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