Multiple endo-beta-1,4-glucanases present in the gut fluid of a defoliating beetle, Podontia quatuordecimpunctata (Coleoptera: Chrysomelidae)
| Autor: | Mohammad Mosleh Uddin, Suman Mojumder, Sikdar Dwaipayan, Mahmudul Hasan Chowdhury |
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| Rok vydání: | 2013 |
| Předmět: |
Protein Denaturation
media_common.quotation_subject Insect Substrate Specificity Beta-1 adrenergic receptor Cellulase Enzyme Stability Animals Zymography Food science media_common chemistry.chemical_classification Larva fungi Temperature Glucanase Hydrogen-Ion Concentration Body Fluids Pupa Coleoptera Gastrointestinal Tract Enzyme chemistry Carboxymethylcellulose Sodium Instar Insect Proteins Agronomy and Crop Science |
| Zdroj: | Pakistan journal of biological sciences : PJBS. 15(7) |
| ISSN: | 1028-8880 |
| Popis: | Endo-beta-1, 4-glucanase (EC 3.2.1.4) activity was measured in the gut fluid of phytophagous insect Podontia quatuordecimpunctata Linnaeus (Coleoptera: Chrysomelidae) in different days of development. The eight day-old larva showed maximum activity with 1.73 U mg(-1) of protein, which was confirmed by gel zymography. In zymogram, using Carboxymethyl Cellulose (CMC) as substrate, four distinct cellulolytic protein bands were detected in leaf borer gut fluids through out of its development. The optimum temperature and pH were 60 degrees C and 5.0, respectively. This endo-beta-1,4-glucanase showed maximum stability at 20-45 degrees C with approximately 20% remaining activity. Zymography also showed complete loss of endo-beta-1,4 glucanase activity at 55 degrees C. This is the first report that the cellulolytic enzyme is produced in the gut of P. quatuordecimpunctata through the whole developmental stages, from the 1st instar to the adult, except for pupae. |
| Databáze: | OpenAIRE |
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