A global analysis of low-complexity regions in the Trypanosoma brucei proteome reveals enrichment in the C-terminus of nucleic acid binding proteins providing potential targets of phosphorylation
| Autor: | Mathieu Cayla, Keith R. Matthews, Alasdair Ivens |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
proteome
Low-complexity regions (LCRs) Medicine (miscellaneous) Computational biology Trypanosoma brucei medicine.disease_cause nucleic acid binding proteins Genome DNA-binding protein General Biochemistry Genetics and Molecular Biology 03 medical and health sciences low-complexity regions (LCRs) Protein targeting Gene expression medicine 030304 developmental biology Regulation of gene expression 0303 health sciences biology phosphorylation 030302 biochemistry & molecular biology RNA Articles liquid-liquid phase separation biology.organism_classification Proteome granules Research Article |
| Zdroj: | Cayla, M, Matthews, K R & Ivens, A C 2020, ' A global analysis of low-complexity regions in the Trypanosoma brucei proteome reveals enrichment in the C-terminus of nucleic acid binding proteins providing potential targets of phosphorylation ', Wellcome Open Research, vol. 5 . https://doi.org/10.12688/wellcomeopenres.16286.2 Wellcome Open Research |
| ISSN: | 2398-502X |
| DOI: | 10.12688/wellcomeopenres.16286.1 |
| Popis: | Background: Low-complexity regions (LCRs) on proteins have attracted increasing attention recently due to their role in the assembly of membraneless organelles or granules by liquid-liquid phase separation. Several examples of such granules have been shown to sequester RNA and proteins in an inactive state, providing an important mechanism for dynamic post-transcriptional gene regulation. In trypanosome parasites, post-transcriptional control overwhelmingly dominates gene regulation due to the organisation of their genome into polycistronic transcription units. The purpose of the current study was to generate a substantially more comprehensive genome-wide survey of LCRs on trypanosome proteins than currently available . Methods: Using the Shannon’s entropy method, provided in the R package ‘entropy’, we identified LCRs in the proteome of Trypanosoma brucei. Our analysis predicts LCRs and their positional enrichment in distinct protein cohorts and superimposes on this a range of post-translational modifications derived from available experimental datasets. Results: We have identified 8162 LCRs present on 4914 proteins, representing 42% of the proteome, placing Trypanosoma brucei among the eukaryotes with the highest percentage of LCRs. Our results highlight the enrichment of LCRs in the C-terminal region of predicted nucleic acid binding proteins, these acting as favoured sites for potential phosphorylation. Phosphorylation represents 51% of the post-translational modifications present on LCRs compared to 16% on the rest of the proteome. Conclusions: The post-translational modifications of LCRs, and in particular phosphorylation events, could contribute to post-transcriptional gene expression control and the dynamics of protein targeting to membraneless organelles in kinetoplastid parasites. |
| Databáze: | OpenAIRE |
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