SARS‐CoV‐2 surveillance in Norway rats ( Rattus norvegicus ) from Antwerp sewer system, Belgium
| Autor: | Natalie Van Houtte, Valeria Carolina Colombo, Lotte Jacobs, Kevin K. Ariën, Arne Iserbyt, Leo Heyndrickx, Marine Hubert, Bram Vanden Broecke, Wannes Leirs, Vincent Sluydts, Herwig Leirs, Joachim Mariën, Peter Delputte, Naomi De Roeck, Sophie Gryseels, Hanne Goris, Joris Elst |
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| Rok vydání: | 2021 |
| Předmět: |
Short Communication
Veterinary medicine viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Antibodies Viral SARS‐CoV‐2 Serology Rodent Diseases Belgium medicine Animals skin and connective tissue diseases Biology Feces Muridae General Veterinary General Immunology and Microbiology biology medicine.diagnostic_test SARS-CoV-2 Transmission (medicine) Pharmacology. Therapy fungi COVID-19 Outbreak General Medicine biology.organism_classification Antibodies Neutralizing Virology Rats respiratory tract diseases body regions Immunoglobulin G Immunoassay disease outbreaks biology.protein RNA Viral Human medicine Antibody |
| Zdroj: | Transboundary and emerging diseases Transboundary and Emerging Diseases |
| ISSN: | 1865-1682 1865-1674 |
| DOI: | 10.1111/tbed.14219 |
| Popis: | SARS-CoV-2 human-to-animal transmission can lead to the establishment of novel reservoirs and the evolution of new variants with the potential to start new out- breaks in humans. We tested Norway rats inhabiting the sewer system of Antwerp, Belgium, for the presence of SARS-CoV-2 following a local COVID-19 epidemic peak. In addition, we discuss the use and interpretation of SARS-CoV-2 serological tests on non-human samples. Between November and December 2020, Norway rat oral swabs, faeces and tissues from the sewer system of Antwerp were collected to be tested by RT-qPCR for the presence of SARS-CoV-2. Serum samples were screened for the presence of anti-SARS-CoV-2 IgG antibodies using a Luminex microsphere immunoas- say (MIA). Samples considered positive were then checked for neutralizing antibodies using a conventional viral neutralization test (cVNT). The serum of 35 rats was tested by MIA showing three potentially positive sera that were later negative by cVNT. All tissue samples of 39 rats analysed tested negative for SARS-CoV-2 RNA. This is the first study that evaluates SARS-CoV-2 infection in urban rats. We can conclude that the sample of rats analysed had never been infected with SARS-CoV-2. However, mon- itoring activities should continue due to the emergence of new variants prone to infect Muridae rodents. |
| Databáze: | OpenAIRE |
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