Visualization of ER-to-Golgi Transport in Living Cells Reveals a Sequential Mode of Action for COPII and COPI
| Autor: | Rainer Pepperkok, Thomas E. Kreis, Suzie J. Scales |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Recombinant Fusion Proteins KDEL Green Fluorescent Proteins Vesicular Transport Proteins Golgi Apparatus Biology Endoplasmic Reticulum Coatomer Protein Microtubules Exocytosis General Biochemistry Genetics and Molecular Biology symbols.namesake Viral Envelope Proteins Chlorocebus aethiops Animals Vero Cells COPII Membrane Glycoproteins Biochemistry Genetics and Molecular Biology(all) Vesicular-tubular cluster Endoplasmic reticulum Cell Membrane Temperature Membrane Proteins Biological Transport COPI COP-Coated Vesicles Golgi apparatus Phosphoproteins Cell Compartmentation Cell biology Luminescent Proteins symbols Carrier Proteins |
| Zdroj: | Cell. 90:1137-1148 |
| ISSN: | 0092-8674 |
| Popis: | Exocytic transport from the endoplasmic reticulum (ER) to the Golgi complex has been visualized in living cells using a chimera of the temperature-sensitive glycoprotein of vesicular stomatitis virus and green fluorescent protein (ts-G-GFPct). Upon shifting to permissive temperature, ts-G-GFPct concentrates into COPII-positive structures close to the ER, which then build up to form an intermediate compartment or transport complex, containing ERGIC-53 and the KDEL receptor, where COPII is replaced by COPI. These structures appear heterogenous and move in a microtubule-dependent manner toward the Golgi complex. Our results suggest a sequential mode of COPII and COPI action and indicate that the transport complexes are ER-to-Golgi transport intermediates from which COPI may be involved in recycling material to the ER. |
| Databáze: | OpenAIRE |
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