Adjuvant activities of crude polysaccharides from cultivated Artemisia rupestris L. on TH1-biased response with the induction of DC activation
| Autor: | Bin Wang, Quanxiao Li, Ailian Zhang, Xiang Weng, Shuangshaung Feng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Low toxicity Traditional medicine medicine.medical_treatment Agriculture (General) Immunology food and beverages RC581-607 Polysaccharide artemisia rupestris l immune response S1-972 Immune system chemistry adjuvant polysaccharide medicine Artemisia rupestris Immunologic diseases. Allergy Agronomy and Crop Science Adjuvant Food Science |
| Zdroj: | Food and Agricultural Immunology, Vol 32, Iss 1, Pp 557-575 (2021) |
| ISSN: | 1465-3443 0954-0105 |
| Popis: | Artemisia rupestris L. polysaccharides have immune enhancement effects with low toxicity in the previous studies. The effect of crude polysaccharides from cultivated Artemisia rupestris L. (CPCAR) on immunity has not been explored. Here, the adjuvant potential of CPCAR was investigated in vivo and its underlying mechanism in vitro was explored through activation of bone marrow-derived dendritic cells (BMDCs). After intramuscular immunizations, CPCAR elevated IgG levels with a TH1-biased response, lymphocyte proliferation and increased CD4 and CD8 T cells in spleen and lymph nodes. IFN-γ and IL-4 of CD8 or CD4 T cells were specifically increased. CPCAR improved DC maturation and diminished Treg frequency. BMDCs treated with CPCAR in vitro showed the up-regulation of CD40, CD86, IL-12 and TNF-α. BMDMs pretreated with TLR4 and NF-kB inhibitors decreased the expressions of CD40 and CD86 and produced less cytokines. These data demonstrated that CPCAR could boost TH1-biased response through DC activation in TLR4/NF-κB signalling. |
| Databáze: | OpenAIRE |
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