Global changes in cellular gene expression during bacteriophage PRD1 infection
| Autor: | Minna M. Poranen, Janne J. Ravantti, Rashi Gupta, A. Marika Grahn, Dennis H. Bamford, Petri Auvinen |
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| Rok vydání: | 2006 |
| Předmět: |
Immunology
Cellular Response to Infection Virus Replication Microbiology Bacteriophage 03 medical and health sciences Virology Gene expression Escherichia coli Bacteriophage PRD1 RNA Messenger Gene 030304 developmental biology Genetics Regulation of gene expression 0303 health sciences biology 030306 microbiology Gene Expression Profiling Polysaccharides Bacterial Virion Gene Expression Regulation Bacterial biology.organism_classification Gene expression profiling Open reading frame Viral replication Genes Bacterial Insect Science Protein Biosynthesis Gene chip analysis sense organs |
| Zdroj: | Journal of virology. 80(16) |
| ISSN: | 0022-538X |
| Popis: | Virus-induced changes in cellular gene expression and host physiology have been studied extensively. Still, there are only a few analyses covering the entire viral replication cycle and whole-host gene pool expression at the resolution of a single gene. Here we report changes in Escherichia coli gene expression during bacteriophage PRD1 infection using microarray technology. Relative mRNA levels were systematically measured for over 99% of the host open reading frames throughout the infection cycle. Although drastic modifications could be detected in the expression of individual genes, global changes at the whole-genome level were moderate. Notably, the majority of virus-induced changes took place only after the synthesis of virion components, indicating that there is no major reprogramming of the host during early infection. The most highly induced genes encoded chaparones and other stress-inducible proteins. |
| Databáze: | OpenAIRE |
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