Avian stem cell factor (SCF): production and characterization of the recombinant His-tagged SCF of chicken and its neutralizing antibody
| Autor: | Bartůnĕk P, Boehmelt G, Beug H, Dvorák M, Stengl G, Martin Zenke, Martin Fh, Pichlíková L |
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| Rok vydání: | 1996 |
| Předmět: |
Erythrocytes
Immunology Molecular Sequence Data Stem cell factor Transfection Biochemistry Polymerase Chain Reaction Antibodies law.invention Cell Line Affinity chromatography law Neutralization Tests Immunology and Allergy Animals Histidine Amino Acid Sequence Progenitor cell Neutralizing antibody Molecular Biology DNA Primers Sequence Tagged Sites Stem Cell Factor biology Cell Differentiation Hematology Hematopoietic Stem Cells Molecular biology Recombinant Proteins Haematopoiesis Proto-Oncogene Proteins c-kit Polyclonal antibodies embryonic structures biology.protein Recombinant DNA Rabbits Antibody Chickens Cell Division Thymidine |
| Zdroj: | Cytokine. 8(1) |
| ISSN: | 1043-4666 |
| Popis: | Recombinant chicken stem cell factor (SCF) was produced in bacteria as a histidine-tagged protein (His delta SCF) and purified by affinity chromatography on an Ni(2+)-NTA agarose column. His delta SCF is devoid of signal peptide and transmembrane domains and therefore mimics a soluble form of SCF. We demonstrate that the factor is biologically fully active in supporting sustained proliferation of SCF-dependent red blood progenitor cells of chicken in vitro. Polyclonal SCF-specific antibodies were raised in rabbits and shown to display potent SCF-neutralizing activity. Thus, recombinant chicken His delta SCF together with the SCF-specific antibody provide valuable tools for studying the role of SCF and c-kit receptor in development, growth and differentiation of avian haematopoietic cells. |
| Databáze: | OpenAIRE |
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