Butyrate response factor 1 is regulated by parathyroid hormone and bone morphogenetic protein-2 in osteoblastic cells
| Autor: | Sjur Reppe, Kaare M. Gautvik, Ole Kristoffer Olstad, Edith Rian, Rune Jemtland, Vigdis T. Gautvik |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Biophysics 8-Bromo Cyclic Adenosine Monophosphate Bone Morphogenetic Protein 2 Parathyroid hormone Biochemistry Bone morphogenetic protein 2 Immediate-Early Proteins Bone remodeling Mice Transforming Growth Factor beta Cell Line Tumor Internal medicine medicine Animals Humans Hedgehog Proteins Receptor Protein kinase A Molecular Biology Cells Cultured Protein kinase C Receptor Parathyroid Hormone Type 1 Osteosarcoma Osteoblasts Chemistry Butyrate Response Factor 1 Zinc Fingers Osteoblast Cell Biology Cyclic AMP-Dependent Protein Kinases Peptide Fragments Rats DNA-Binding Proteins Endocrinology medicine.anatomical_structure Gene Expression Regulation Parathyroid Hormone Bone Morphogenetic Proteins Trans-Activators Receptors Parathyroid Hormone |
| Zdroj: | Biochemical and Biophysical Research Communications. 324:218-223 |
| ISSN: | 0006-291X |
| Popis: | Parathyroid hormone (PTH) exerts potent and diverse effects in bone and cartilage through activation of type 1 PTH receptors (PTH1R) capable of coupling to protein kinase A (PKA) and PKC. We have used macroarrays to identify zinc finger protein butyrate response factor-1 (BRF1) as a novel PTH regulated gene in clonal and normal osteoblasts of human and rodent origin. We further demonstrate that in human osteoblast-like OHS cells, biologically active hPTH(1–84) and hPTH(1–34) stimulate BRF1 mRNA expression in a dose- and time-dependent manner, while the amino-terminally truncated hPTH(3–84) which does not activate PTH1R has no effect. Moreover, using specific stimulators or inhibitors of PKA and PKC activity, the PTH-elicited BRF1 mRNA expression is mediated through the PKA signaling pathway. In mouse calvarial osteoblasts, BRF1 mRNA levels are upregulated by PTH(1–84) and reduced in response to bone morphogenetic protein 2 (BMP-2). Hence, our data showing that BRF1 is expressed in osteoblastic cells and regulated by PTH and BMP-2, suggest an important role for BRF1 in osteoblasts within the molecular network of PTH-dependent bone remodeling. |
| Databáze: | OpenAIRE |
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