Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation
| Autor: | Helmut Ruis, Gerald Cohen, Joanna Rytka, Aleksandra Traczyk, Friederike Fessl |
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| Rok vydání: | 1985 |
| Předmět: |
Genotype
Saccharomyces cerevisiae Mutant Genes Fungal chemistry.chemical_compound Species Specificity Transcription (biology) Genetics Escherichia coli Cloning Molecular DNA Fungal Molecular Biology Gene Alleles biology Structural gene Genetic Complementation Test Nucleic Acid Hybridization biology.organism_classification Catalase Molecular biology Complementation chemistry Genes Mutation biology.protein DNA Plasmids |
| Zdroj: | Moleculargeneral genetics : MGG. 200(1) |
| ISSN: | 0026-8925 |
| Popis: | As a first step in an analysis of the DNA regions involved in the control of the catalase A gene of Saccharomyces cerevisiae by glucose, heme, and oxygen this gene has been cloned. Catalase A-deficient mutants were obtained by UV mutagenesis of a ctt1 mutant strain specifically lacking catalase T. All the catalase A-deficient mutants obtained fall into one complementation group. The single recessive mutation causing specific lack of catalase A was designated cta1. Several overlapping DNA fragments complementing the cta1 mutation were obtained by transforming ctt1 cta1 double mutants with a yeast gene library in vector YEp13. Hybrid selection of RNA with the help of one of the cloned DNAs followed by in vitro translation of this RNA and identification of the protein synthesized with catalase A-specific antibodies showed that the catalase A structural gene has been cloned. A single copy of this gene is present in the yeast genome. Transcription of the catalase A gene cloned into vector YEp13 is repressed by glucose. The DNA isolated hybridizes to a 1.6 kb polyA+−RNA virtually absent from heme-deficient cells, presumably catalase A mRNA. |
| Databáze: | OpenAIRE |
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