Polymorphisms of the CTLA-4 exon 1 and promoter gene in systemic lupus erythematosus
| Autor: | G. G. Song, Young Hen Lee, Jong Dae Ji, Jeongwon Sohn, Ye Ree Kim |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Immunoconjugates Adolescent Genotype T-Lymphocytes 030204 cardiovascular system & hematology Gastroenterology law.invention Abatacept 03 medical and health sciences Exon 0302 clinical medicine Rheumatology Gene Frequency law Antigens CD Internal medicine medicine Humans Lupus Erythematosus Systemic CTLA-4 Antigen Promoter Regions Genetic Allele frequency Polymerase chain reaction Autoantibodies 030203 arthritis & rheumatology Lupus erythematosus Systemic lupus erythematosus Polymorphism Genetic business.industry Autoantibody Promoter Exons Middle Aged medicine.disease Antigens Differentiation Phenotype Immunology Female business |
| Zdroj: | Lupus. 10(9) |
| ISSN: | 0961-2033 |
| Popis: | The objective of this study was to determine whether the polymorphisms of the CTLA-4 exon 1 (/49) and promoter (7318) are associated with susceptibility to systemic lupus erythematosus (SLE) and its clinical features. Polymerase chain reaction of genomic DNA-restriction fragment length polymorphism was used to determine genotypes of the CTLA-4 exon 1 (/49) and promoter (7318) in 80 SLE patients and 86 healthy control subjects. The genotype distribution of the CTLA-4 exon 1 (/49) differed between SLE patients and controls (w2= 6.74, 2 degrees of freedom (d.f.), P = 0.03). The CTLA-4 AG genotype occurred more frequently in patients with SLE (46.3% vs 33.7% controls). On the other hand, the CTLA-4 AA genotype as well as the CTLA-4 GG genotype was less frequent among SLE patients than among control subjects (1.3% vs 9.3% and 52.5% vs 57.0%, respectively). The genotype distribution of the CTLA-4 promoter (-318) different between SLE patients and control subjects (CT, TT, CC; genotype 27.5%, 0%, 72.5% vs 16.3%, 4.7%, 79.1% controls respectively, w2= 6.36 2 d.f., P = 0.04). However, Fisher's exact or w2P-values for each genotypes of the CTLA-4 exon l (+49) and promoter (-318) between SLE and control group < 0.05. Clinically, in the lupus patients there was no significant difference according to the CTLA-4 polymorphism.In conclusion, no correlation was found between CTLA-4 exon 1 (+49) and promoter (-318) polymorphisms and SLE in our study. |
| Databáze: | OpenAIRE |
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