The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of Caenorhabditis elegans
| Autor: | Cheng-Gang Zou, Yan-Lu Luo, Xin Li, Ke-Qin Zhang, Lianming Liang, Ying-Qian Kang |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Virulence Factors Mutant Hyphae Virulence 01 natural sciences Applied Microbiology and Biotechnology Microbiology Virulence factor Nematophagous fungus Fungal Proteins 03 medical and health sciences Ascomycota Animals Caenorhabditis elegans Serine protease chemistry.chemical_classification Reactive oxygen species NADPH oxidase biology NADPH Oxidases General Medicine Spores Fungal biology.organism_classification Cell biology 030104 developmental biology chemistry Mutation biology.protein Serine Proteases Reactive Oxygen Species Oxidation-Reduction 010606 plant biology & botany |
| Zdroj: | Journal of microbiology (Seoul, Korea). 55(11) |
| ISSN: | 1976-3794 |
| Popis: | Reactive oxygen species (ROS) produced by NADPH oxidases can serve as signaling molecules to regulate a variety of physiological processes in multi-cellular organisms. In the nematophagous fungus Arthrobotrys oligospora, we found that ROS were produced during conidial germination, hyphal extension, and trap formation in the presence of nematodes. Generation of an AoNoxA knockout strain demonstrated the crucial role of NADPH oxidase in the production of ROS in A. oligospora, with trap formation impaired in the AoNoxA mutant, even in the presence of the nematode host. In addition, the expression of virulence factor serine protease P186 was up-regulated in the wild-type strain, but not in the mutant strain, in the presence of Caenorhabditis elegans. These results indicate that ROS derived from AoNoxA are essential for full virulence of A. oligospora in nematodes. |
| Databáze: | OpenAIRE |
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