Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection
Autor: | Rawleigh Howe, Liya Wassie, Søren T. Hoff, Markos Abebe, Tamrat Abebe, Birhan Tamene Alemnew, Abraham Aseffa |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine lcsh:Immunologic diseases. Allergy Tuberculosis Adolescent mRNA 030106 microbiology Immunology Biology 03 medical and health sciences Latent Tuberculosis Immunity TLR medicine Humans RNA Messenger Child Receptor Gene Children Messenger RNA Innate immune system Latent tuberculosis Research Toll-Like Receptors Mycobacterium tuberculosis medicine.disease Immunity Innate Fold change Early Diagnosis 030104 developmental biology Latency Female lcsh:RC581-607 Biomarkers |
Zdroj: | BMC Immunology, Vol 22, Iss 1, Pp 1-9 (2021) BMC Immunology |
ISSN: | 1471-2172 |
Popis: | Background Understanding immune mechanisms, particularly the role of innate immune markers during latent TB infection remains elusive. The main objective of this study was to evaluate mRNA gene expression patterns of toll-like receptors (TLRs) as correlates of immunity during latent TB infection and further infer their roles as potential diagnostic biomarkers. Methods Messenger RNA (mRNA) levels were analysed in a total of 64 samples collected from apparently healthy children and adolescents latently infected with tuberculosis (n = 32) or non-infected (n = 32). Relative expression in peripheral blood of selected genes encoding TLRs (TLR-1, TLR-2, TLR-4, TLR-6 and TLR-9) was determined with a quantitative real-time polymerase chain reaction (qRT-PCR) using specific primers and florescent labelled probes and a comparative threshold cycle method to define fold change. Data were analysed using Graph-Pad Prism 7.01 for Windows and a p-value less than 0.05 was considered statistically significant. Results An increased mean fold change in the relative expression of TLR-2 and TLR-6 mRNA was observed in LTBI groups relative to non-LTBI groups (p Conclusions An increased mRNA expression of TLR-2 and TLR-6 was observed in latently infected individuals relative to those non-infected, possibly indicating the roles these biomarkers play in sustenance of the steady state interaction between the dormant TB bacilli and host immunity. |
Databáze: | OpenAIRE |
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