PHM 14: A novel monoclonal antibody that reacts with both normal and neoplastic human B cells but not B-CLL
| Autor: | I. G. Barr, N. Kraft, Robert C. Atkins, B. H. Toh, Wayne W. Hancock |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
B-Lymphocytes
Leukemia Lymphoma medicine.drug_class T cell Antibodies Monoclonal Fluorescent Antibody Technique Hematology Biology Monoclonal antibody Binding Competitive Molecular biology Cell Line Immunoenzyme Techniques medicine.anatomical_structure Polyclonal antibodies hemic and lymphatic diseases biology.protein medicine Humans Lymph Nodes Antibody Spleen B cell |
| Zdroj: | Scandinavian Journal of Haematology. 33:187-196 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1984.tb02395.x |
| Popis: | The reactivity of PHM 14, a monoclonal antibody to B cells produced in our laboratory was compared with Leu 10, a commercially produced antibody. Flow microfluorometric analysis showed that both PHM 14 and Leu 10 reacted with normal B cells, a variety of B cell lines and with B cell leukaemias and lymphomas, except in the case of B-CLL where PHM 14 reacted with a minority of cases, whereas Leu 10 reacted with a majority. PHM 14 and Leu 10 did not react with normal monocytes, granulocytes, T cells, T cell lines or with T cell leukaemias or lymphomas. Specificity of PHM 14 for B cells was further supported by the demonstration that PHM 14-positive blood cells, sorted from PHM 14-negative blood cells, reacted with polyclonal anti-immunoglobulins and with a panel of B cell markers but did not react with markers for T cells, monocytes, granulocytes or NK cells. Our results suggest that both PHM 14 and Leu 10 should be useful diagnostic reagents in the investigation of B cell-derived leukaemias and lymphomas. |
| Databáze: | OpenAIRE |
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