Inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits
| Autor: | Beatriz Establés-Ortiz, Luis González-Candelas, Ana-Rosa Ballester, Paco Romero, María T. Lafuente |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia y Tecnología (España), Generalitat Valenciana, Consejo Superior de Investigaciones Científicas (España), European Commission |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cyclopropanes Ethylene Physiology Cell Respiration Plant Science Protein degradation medicine.disease_cause 1-Methylcyclopropene Nitric Oxide 01 natural sciences Nitric oxide Xenobiotics 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Respiration Genetics medicine Mode of action Plant Proteins chemistry.chemical_classification Reactive oxygen species Cyanides Non-climacteric fruits Ethylenes 030104 developmental biology chemistry Biochemistry 1-Methylcyclopropene (1-MCP) Oxidative stress Fruit Reactive Oxygen Species 010606 plant biology & botany Citrus sinensis |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | The ethylene perception inhibitor 1-methylcyclopropene (1-MCP) has been critical in understanding the hormone's mode of action. However, 1-MCP may trigger other processes that could vary the interpretation of results related until now to ethylene, which we aim to understand by using transcriptomic analysis. Transcriptomic changes in ethylene and 1-MCP-treated ‘Navelate’ (Citrus sinensis L. Osbeck) oranges were studied in parallel with changes in ethylene production, respiration and peel damage. The effects of compounds modifying the levels of the ethylene co-product cyanide and nitric oxide (NO) on fruit physiology were also studied. Results suggested that: 1) The ethylene treatment caused sub-lethal stress since it induced stress-related responses and reduced peel damage; 2) 1-MCP induced ethylenedependent and ethylene-independent responsive networks; 3) 1-MCP triggered ethylene overproduction, stress-related responses and metabolic shifts aimed to cope with cell toxicity, which mostly affected to the inner part of the peel (albedo); 4) 1-MCP increased respiration and drove metabolism reconfiguration for favoring energy conservation but up-regulated genes related to lipid and protein degradation and triggered the over-expression of genes associated with the plasma membrane cellular component; 5) Xenobiotics and/or reactive oxygen species (ROS) might act as signals for defense responses in the ethylene-treated fruit, while their uncontrolled generation would induce processes mimicking cell death and damage in 1-MCP-treated fruit; 6) ROS, the ethylene co-product cyanide and NO may converge in the toxic effects of 1-MCP. This work was supported by the Spanish Ministry of Science and Technology (Research Grants AGL2002-1727 and AGL2009-11969 and by the Generalitat Valenciana, Spain (Grant PROMETEOII/2014/027). Dr. B. E. was the recipient of a fellowship of the Spanish Ministry of Science and Technology. Dr. A.R.B. is grateful to CSIC and the European Social Fund for her postdoctoral JAE-Doc contract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|