Characterization of the DNA- and Metal-Binding Properties of Vibrio anguillarum Fur Reveals Conservation of a Structural Zn 2+ Ion
| Autor: | Richard G. Brennan, Ekaterina E. Zheleznova, Jorge H. Crosa |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
inorganic chemicals
DNA Bacterial Siderophore Vibrio anguillarum animal diseases Molecular Sequence Data Repressor Plasma protein binding medicine.disease_cause Microbiology chemistry.chemical_compound Bacterial Proteins Nickel Escherichia coli medicine Amino Acid Sequence Molecular Biology Vibrio Manganese integumentary system biology Cobalt biology.organism_classification Enzymes and Proteins Molecular biology Repressor Proteins Zinc Biochemistry chemistry Genes Bacterial bacteria Ferric Sequence Alignment DNA Cadmium Protein Binding medicine.drug |
| Zdroj: | Journal of Bacteriology. 182:6264-6267 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.182.21.6264-6267.2000 |
| Popis: | The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn 2+ , Co 2+ , Cd 2+ , and to a lesser extent Ni 2+ but, unlike E. coli Fur, not in the presence of Zn 2+ . We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding. |
| Databáze: | OpenAIRE |
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