Dried blood spot punches for confirmation of suspected γ-hydroxybutyric acid intoxications: validation of an optimized GC–MS procedure
| Autor: | Ann-Sofie M. E. Ingels, Peter De Paepe, Kurt Anseeuw, Diederik Van Sassenbroeck, Christophe P. Stove, Hugo Neels, Willy E. Lambert |
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| Rok vydání: | 2011 |
| Předmět: |
Clinical Biochemistry
Analytical chemistry Hydroxybutyrates Hematocrit behavioral disciplines and activities Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound γ-Hydroxybutyric acid Lc ms ms Medicine and Health Sciences WHOLE-BLOOD Humans Medicine TANDEM MASS-SPECTROMETRY LC-MS/MS General Pharmacology Toxicology and Pharmaceutics skin and connective tissue diseases Derivatization Dried blood Biology Whole blood Chromatography medicine.diagnostic_test business.industry Pharmacology. Therapy HUMANS General Medicine Dried blood spot Chemistry Medical Laboratory Technology surgical procedures operative chemistry Human medicine Dried Blood Spot Testing HEMATOCRIT Gas chromatography–mass spectrometry GHB business |
| Zdroj: | BIOANALYSIS Bioanalysis |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.11.204 |
| Popis: | Background: γ-hydroxybutyric acid ( GHB ), notorious as a club- and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by ‘on-spot’ derivatization and analysis by GC–MS. Results: A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 20–35 µl spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2–100 µg/ml. Conclusion: A sensitive GC–MS method for GHB analysis in DBS was successfully optimized and validated. The successful analysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB analysis in toxicological cases. |
| Databáze: | OpenAIRE |
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