Time-dependent increase in rat mammary uridine diphosphate glucose 4-epimerase activity in vitro
| Autor: | K.E. Ebner, D.K. Fitzgerald, E. Richardson |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Glycerol
Time Factors Uracil Nucleotides Guinea Pigs Bile Acids and Salts chemistry.chemical_compound Fluorides Mammary Glands Animal Pregnancy Lactation Chiroptera Cricetinae medicine Animals Urea Sodium Cholate Isomerases Incubation chemistry.chemical_classification General Medicine Hydrogen-Ion Concentration NAD In vitro Rats Enzyme Activation Molecular Weight Kinetics medicine.anatomical_structure Enzyme Glucose Marsupialia chemistry Biochemistry Cats Female NAD+ kinase Rabbits NADP |
| Zdroj: | Biochimica et biophysica acta. 206(2) |
| ISSN: | 0006-3002 |
| Popis: | UDP-glucose 4-epimerase (EC 5.1.3.2) activity increase upon incubation of extracts prepared from lactating mammary glands. The increase in activity is enhanced by the addition of NAD+, NADP+, UTP, UDP, UMP and glycerol to the extracts. The increase in enzymatic activity is influenced by pH and urea concentration but not by the removal of low molecular weight material. The increase in activity is not influenced by 1.0 mM phenylmethylsulfonylfluoride or 1% sodium cholate. The “activation” process does not alter the K m for UDP-galactose. The present evidence indicates that the increase in activity is due to a process leading to a more active form of enzyme. |
| Databáze: | OpenAIRE |
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