Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation?
| Autor: | Patricia Sousa Dantas, Tatiana Sousa Cunha, Vinicius Guzzoni, Juliana Dinéia Perez, Maisa Mayumi Sakata, Dulce Elena Casarini, Fernando César Bizerra, Vander José das Neves, Fernanda Klein Marcondes, Regina Lúcia Harumi Watanabe |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty High-intensity resistance training Proteome Heart Ventricles Hemodynamics Proteomics shotgun Biology Ventricular Function Left General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Physical Conditioning Animal Internal medicine medicine Animals Rats Wistar General Pharmacology Toxicology and Pharmaceutics Pathological Maladaptation Cardioprotection Regulation of gene expression Myocardium Resistance Training General Medicine medicine.disease Adaptation Physiological Rats Rat proteomics Cardiac hypertrophy 030104 developmental biology medicine.anatomical_structure Endocrinology Gene Expression Regulation Ventricle Heart failure Hypertrophy Left Ventricular Pathological hypertrophy |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| Popis: | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP FAEP/UNICAMP CAPES, Brazil High-intensity resistance training (RT) induces adaptations that improve physiological function. However, high intensity, volume and/or frequency may lead to injury and other health issues such as adverse cardiac effects. The aim of this study was to evaluate the effect of RT on left ventricle proteome, and to identify the pathways involved on the harmful adaptations induced by this protocol. Male Wistar rats were randomized into 2 groups: Trained (T) and Sedentary (S). Animals from T group were trained for 6 weeks, and then all the animals were sacrificed and left ventricle was isolated for analysis. We identified 955 proteins, and 93 proteins were considered 36 were expressed exclusively in T group, and 4 in S group. Based on quantitative analysis, 42 proteins were found overexpressed and 11 underexpressed in T group compared with S group. Using the Gene Ontology to relate the biological processes in which these proteins are involved, we conclude that RT protocol promotes changes similar to those found in the initial phase of heart failure, but we also observed a concomitant increased expression of protective proteins, suggesting the activation of pathways to avoid major damages on left ventricle and delay the onset of pathological hypertrophy. Statement of significance of the study: Our study shows that high-intensity RT protocol changes left ventricle proteome, modifying metabolic profile of heart tissue and inducing the expression of proteins that acts against cardiac injury. We hypothesize that these adaptations occur to prevent the onset of cardiac dysfunction. Despite highly significant, it remains to be determined whether these adaptations are sufficient to further keep left ventricle function and exert cardioprotection, and whether this panel will be shifted towards maladaptation, and heart failure. (c) 2016 Elsevier Inc. All rights reserved. Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Nephrol, Sao Paulo, SP, Brazil Fed Univ Sao Paulo UNIFESP, Dept Physiol, Discipline Nutr Physiol, Sao Paulo, SP, Brazil Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, Special Mycol Lab LEMI, Sao Paulo, SP, Brazil Univ Estadual Campinas, Fac Dent Piracicaba FOP UNICAMP, Dept Physiol Sci, Piracicaba, SP, Brazil Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, BR-12231280 Sao Jose Dos Campos, SP, Brazil Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Nephrol, Sao Paulo, SP, Brazil Fed Univ Sao Paulo UNIFESP, Dept Physiol, Discipline Nutr Physiol, Sao Paulo, SP, Brazil Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, Special Mycol Lab LEMI, Sao Paulo, SP, Brazil Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, BR-12231280 Sao Jose Dos Campos, SP, Brazil FAPESP: 02/05427-8 FAEP/UNICAMP: 398/03 FAEP/UNICAMP: 680/03 Web of Science |
| Databáze: | OpenAIRE |
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