Foot-and-mouth disease virus infection stimulates innate immune signaling in the mouse macrophage RAW 264.7 cells
| Autor: | Yanquan Wei, Ping Du, Chang Yanyan, Xiaoying Zhi, Yun Zhang, Huichen Guo, Gao Yuan, Run Wu, Jianliang Lv |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype viruses animal diseases Immunology Biology Applied Microbiology and Biotechnology Microbiology Virus Cell Line Mice 03 medical and health sciences Gene expression Genetics Animals Secretion Molecular Biology RAW 264.7 Cells Cytopathic effect Innate immune system Microarray analysis techniques Macrophages virus diseases General Medicine biochemical phenomena metabolism and nutrition Immunity Innate 030104 developmental biology Gene Expression Regulation Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Cytokines Interferons Transcriptome Signal Transduction |
| Zdroj: | Canadian Journal of Microbiology. 64:155-166 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | The innate immune system acts as the first line of defense against invasion by bacterial and viral pathogens. The role of macrophages in innate immune responses to foot-and-mouth disease virus (FMDV) is poorly understood. To determine the mechanism underlying activation of innate immunity after FMDV infection in macrophages, we performed FMDV infection in mouse macrophage RAW 264.7 cells and found that FMDV serotype O infection induced a cytopathic effect. We then evaluated the gene expression profile in macrophage RAW 264.7 cells after FMDV infection using systematic microarray analysis. Gene ontology annotation and enrichment analysis revealed that FMDV promoted expression in a group of genes that are enriched in innate immune response and inflammatory response processes. Further research demonstrated that FMDV serotype O infection enhanced NF-κB, Toll-like, and RIG-I-like receptor signaling pathways and proteins expression and increased transcription and expression of a series of cytokines and interferons, as proved by qRT-PCR, Western blot, ELISA, and dual-luciferase reporter assay. Our study concluded that FMDV infection triggers the innate immune response in macrophages after activation of multiple innate immune pathway receptors and proteins by FMDV serotype O, resulting in activation and secretion of a series of cytokines and interferons. |
| Databáze: | OpenAIRE |
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