Generation of induced pluripotent stem cell lines ICGi021-A and ICGi022-A from peripheral blood mononuclear cells of two healthy individuals from Siberian population
| Autor: | J.A. Rzaev, A.A. Malakhova, T.B. Malankhanova, E.A. Khabarova, K.R. Valetdinova, S. V. Pavlova, Suren M. Zakian, Yuri Vyatkin, Sergey P. Medvedev, E.V. Grigor'eva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cellular differentiation Induced Pluripotent Stem Cells Germ layer Biology Peripheral blood mononuclear cell Cell Line 03 medical and health sciences 0302 clinical medicine Humans Induced pluripotent stem cell lcsh:QH301-705.5 Exome sequencing Karyotype Cell Differentiation Cell Biology General Medicine Cellular Reprogramming Molecular biology Siberia 030104 developmental biology lcsh:Biology (General) Cell culture Leukocytes Mononuclear Female Reprogramming 030217 neurology & neurosurgery Germ Layers Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 48, Iss, Pp 101952-(2020) |
| ISSN: | 1873-5061 |
| Popis: | ICGi021-A and ICGi022-A iPSC lines were obtained by reprogramming PBMCs of two healthy women of the Siberian population using episomal non-integrating vectors expressing Yamanaka factors. iPSC lines expressed pluripotency markers, had a normal karyotype and demonstrated the ability to differentiate into derivatives of the three germ layers. Clinical exome sequencing data of the original biosamples of the donors are available in the NCBI SRA database. The generated cell lines are useful as "healthy" control in biomedical studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|