Expression levels of sonic hedgehog pathway genes and their targets are upregulated in early clear cell renal cell carcinoma
| Autor: | Anna Kotulak‑Chrzaszcz, Agnieszka Rybarczyk, Jakub Klacz, Marcin Matuszewski, Zbigniew Kmiec, Piotr Wierzbicki |
|---|---|
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Molecular Medicine. 49 |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2022.5114 |
| Popis: | Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive subtype of kidney cancer, with high mortality rates worldwide. The sonic hedgehog (SHH) molecular cascade is altered in various malignancies in tumorigenesis, and several SHH pathway inhibitors have been considered as potential anticancer drugs. The aim of the present study was to determine the expression profile of SHH signaling components and their target genes in ccRCC. Additionally, the present study examined the effects of SHH pathway inhibitory drugs (RU‑SKI43, cyclopamine and GLI‑antagonist 61) on cell viability, cell cycle progression, expression levels of SHH target genes and migration ability in 786‑O, ACHN and HK2 cells. The study also included paired tumor and normal samples from 62 patients with ccRCC. The mRNA levels in clinical samples and cell lines were measured via reverse transcription‑quantitative PCR. Cell viability was examined using a sulforhodamine B assay. Flow cytometry was used to investigate cell cycle progression and the migratory rate of cells was assessed using a wound healing assay. High mRNA levels of |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|