Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes
| Autor: | Morag Livingstone, Ewan M. Clark, David Longbottom, Lucy Spalding, Michelle Sait, Frederick A Lainson, Nick Wheelhouse, Bryan Markey, Garry S. A. Myers, Simone Magnino |
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| Jazyk: | angličtina |
| Předmět: |
Genome
Feces Plasticity zone Synovial Fluid Chlamydia pecorum Chlamydia Phylogeny 2. Zero hunger Genetics 0303 health sciences Cytotoxins Structural gene Tryptophan High-Throughput Nucleotide Sequencing QR Microbiology 3. Good health 579 Microorganisms fungi & algae Tandem Repeat Sequences Polymorphic membrane proteins Research Article Biotechnology Bioinformatics Sequence analysis Hypothetical protein Molecular Sequence Data Chlamydiae Biology 03 medical and health sciences Folic Acid Bacterial Proteins Animals Humans Clustered tandem repeats Folate biosynthesis Tryptophan metabolism Gene 030304 developmental biology Whole genome sequencing Chlamydia pecorum Genome sequence Polymorphic membrane proteins Plasticity zone Tryptophan metabolism Folate biosynthesis Clustered tandem repeats Sheep Base Sequence 030306 microbiology Membrane Proteins Sequence Analysis DNA biology.organism_classification Genome sequence Cattle Caco-2 Cells Genome Bacterial |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/1471-2164-15-23 |
| Popis: | Background Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the synovial fluid of a sheep with polyarthritis (strain P787) and from a cervical swab taken from a cow with metritis (strain PV3056/3) were determined using Illumina/Solexa and Roche 454 genome sequencing. Results Gene order and synteny was almost identical between C. pecorum strains and C. psittaci. Differences between C. pecorum and other chlamydiae occurred at a number of loci, including the plasticity zone, which contained a MAC/perforin domain protein, two copies of a >3400 amino acid putative cytotoxin gene and four (PV3056/3) or five (P787 and W73) genes encoding phospholipase D. Chlamydia pecorum contains an almost intact tryptophan biosynthesis operon encoding trpABCDFR and has the ability to sequester kynurenine from its host, however it lacks the genes folA, folKP and folB required for folate metabolism found in other chlamydiae. A total of 15 polymorphic membrane proteins were identified, belonging to six pmp families. Strains possess an intact type III secretion system composed of 18 structural genes and accessory proteins, however a number of putative inc effector proteins widely distributed in chlamydiae are absent from C. pecorum. Two genes encoding the hypothetical protein ORF663 and IncA contain variable numbers of repeat sequences that could be associated with persistence of infection. Conclusions Genome sequencing of three C. pecorum strains, originating from animals with different disease manifestations, has identified differences in ORF663 and pseudogene content between strains and has identified genes and metabolic traits that may influence intracellular survival, pathogenicity and evasion of the host immune system. Electronic supplementary material The online version of this article (doi:10.1186/1471-2164-15-23) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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