Selective effects of whey protein concentrate on glutathione levels and apoptosis in rats with mammary tumors
| Autor: | Yang-Ming Tseng, Shih-Hsuan Cheng, Li-Yu Tsai, Shih-Meng Tsai, Szu-Hsien Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Whey protein Antioxidant medicine.medical_treatment DMBA Apoptosis Breast Neoplasms Biology Toxicology medicine.disease_cause Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Humans bcl-2-Associated X Protein Caspase 3 7 12-Dimethylbenz[a]anthracene General Medicine Glutathione Rats Disease Models Animal Oxidative Stress Whey Proteins 030104 developmental biology Endocrinology Liver chemistry 030220 oncology & carcinogenesis Cancer cell Female Carcinogenesis Food Science |
| Zdroj: | Food and Chemical Toxicology. 107:440-448 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2017.07.024 |
| Popis: | Glutathione (GSH) plays an important role in antioxidant defense and regulation of apoptosis. GSH deficiency is related to many diseases, including cancer, and increased GSH levels in cancer cells are associated with chemotherapy resistance because of resistance to apoptosis. In this study, we investigated the effects of whey protein concentrate (WPC), a precursor of GSH, in rats with mammary tumors induced by treatment with 7,12-dimethylbenz(a)anthracene (DMBA). DMBA treatment results in cellular changes that mimic the initiation and promotion of carcinogenesis of breast tissue. We aimed to examine the possible preventive effects of diets containing whey protein on DMBA-induced mammary tumors in rats. The results indicate that WPC (0.334 g/kg) supplementation significantly increased the liver GSH levels by 92%, and were accompanied by low Bax/Bcl-2 ratio (from 5 to 3) and cleaved caspase-3/procaspase-3 ratio (from 2.4 to 1.2) in DMBA-treated rats. Furthermore, tumor GSH levels were decreased by 47% in WPC-supplemented rats, which resulted in increased Bax/Bcl-2 ratio (from 0.9 to 2) and cleaved caspase-3/procaspase-3 ratio (from 1.1 to 2.7). In conclusion, supplementation with WPC could selectively deplete tumor GSH levels and, therefore, WPC supplementation might be a promising strategy to overcome treatment resistance in cancer therapy. |
| Databáze: | OpenAIRE |
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