Antibodies to HIV integrase catalyze site-specific degradation of their antigen
| Autor: | Valentina N. Buneva, Olga D. Zakharova, Marie-Line Andreola, Valeriy A. Rasskazov, Georgy A. Nevinsky, Elena S. Odintsova, Vincent Parissi, Pavel S. Dmitrenok, Christina Calmels, Svetlana V. Baranova |
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| Přispěvatelé: | Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2011 |
| Předmět: |
Male
HIV Antigens HIV Infections HIV Integrase HIV Antibodies Substrate Specificity Immunology and Allergy MESH: Immunoglobulin G 0303 health sciences Oligopeptide Chymotrypsin biology 030302 biochemistry & molecular biology Proteolytic enzymes General Medicine MESH: HIV Infections Trypsin MESH: Immunoglobulin M 3. Good health Integrase [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry MESH: Young Adult Female MESH: Biocatalysis medicine.drug Adult Proteases Adolescent Immunology 03 medical and health sciences Young Adult Affinity chromatography medicine Humans 030304 developmental biology MESH: Adolescent MESH: Humans MESH: HIV Antibodies MESH: Adult Proteinase K Molecular biology MESH: Male Immunoglobulin M Immunoglobulin G biology.protein Biocatalysis MESH: Substrate Specificity MESH: HIV Integrase MESH: Female MESH: HIV Antigens |
| Zdroj: | International Immunology International Immunology, Oxford University Press (OUP), 2011, 23 (10), pp.601-12. ⟨10.1093/intimm/dxr065⟩ |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxr065⟩ |
| Popis: | International audience; HIV-1 integrase (IN) catalyzes integration of a DNA copy of the viral genome into the host genome. In contrast to canonical proteases (trypsin, chymotrypsin and proteinase K), IgGs and IgMs isolated from HIV-infected patients by affinity chromatography on immobilized IN specifically hydrolyzed only IN but not many other tested intact globular proteins. The sites of IN cleavage determined by MALDI mass spectrometry were localized mainly within seven known immunodominant regions of IN. Thin layer chromatography analysis has shown that the abzymes (Abzs) could also cleave 17 to 22-mer oligopeptides (OPs) corresponding to the immunodominant regions of IN sequence with a much higher rate than non-specific long peptides or three- and tetrapeptides of various sequence. Therefore, a prolonged incubation of IN with AIDS IgGs and IgMs having high catalytic activity usually produces many OPs of different length. Since anti-IN IgGs and IgMs can efficiently hydrolyze IN, a positive role of the Abzs in counteracting the infection is possible. |
| Databáze: | OpenAIRE |
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