Pertussis toxin and H-7 distinguish mechanisms involved in eicosanoid release from lipopolysaccharide-primed macrophages. Eicosanoid release from lipopolysaccharide-primed macrophages
| Autor: | G. Larry Cottam, Akira Matsunaga, Bonnie C. Miller |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Lipopolysaccharides
Bordetella pertussis Lipopolysaccharide Arachidonic Acids Biology Pertussis toxin Biochemistry Dinoprostone Piperazines Microbiology Mice chemistry.chemical_compound 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Animals Virulence Factors Bordetella Calcimycin Cells Cultured Protein kinase C Mice Inbred BALB C Leukotriene Macrophages Macrophage Activation Isoquinolines biology.organism_classification Pertussis Toxin Eicosanoid chemistry Phorbol Eicosanoids Tetradecanoylphorbol Acetate Female SRS-A lipids (amino acids peptides and proteins) |
| Zdroj: | European Journal of Biochemistry. 187:599-603 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1990.tb15342.x |
| Popis: | Release of eicosanoids is an important response of macrophages to inflammation and bacterial infection. At low concentrations, bacterial lipopolysaccharide (1-2 micrograms/ml) fails to stimulate eicosanoid release in resident peritoneal macrophages but primes the macrophages for a greatly enhanced release of eicosanoids on stimulation with the calcium ionophore A23187 (0.1 microM) or with phorbol 12-myristate 13-acetate (50 nM), an activator of protein kinase C. Incubation of macrophages with Bordetella pertussis toxin, prior to priming with lipopolysaccharide, inhibited the release of both cyclooxygenase and lipoxygenase products upon A23187 stimulation. Pertussis toxin treatment of macrophages had no effect on eicosanoid release when the stimulus was phorbol 12-myristate 13-acetate. The presence of 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), an effective inhibitor of protein kinase C, during lipopolysaccharide priming and subsequent stimulation significantly inhibited eicosanoid release when phorbol 12-myristate 13-acetate was the stimulus, but did not affect eicosanoid release stimulated by A23187. Based on these results, at least two mechanisms, distinguished by apparent differences in sensitivity to pertussis-toxin-sensitive, guanine-nucleotide-binding proteins and protein kinase C, are involved in eicosanoid secretion by lipopolysaccharide-activated macrophages in response to A23187 and phorbol 12-myristate 13-acetate. |
| Databáze: | OpenAIRE |
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