Putative biomarkers for malignant pleural mesothelioma suggested by proteomic analysis of cell secretome

Autor: Alfonso Cristaudo, Maria Rosa Mazzoni, Viviana Greco, Rudy Foddis, Alessandra Bonotti, Vanessa D'Antongiovanni, Luisa Pieroni, Federica Ciregia, Gino Giannaccini, Laura Giusti, Serena Lacerenza, Poupak Fallahi, Antonio Lucacchini
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Mesothelioma
Cancer Research
Lung Neoplasms
Proteome
Cell
Malignant pleural mesothelioma
cell lines
Biochemistry
Saposins
0302 clinical medicine
Neoplasm
Oxidoreductases Acting on Sulfur Group Donors
Gel electrophoresis
Secretory Pathway
biology
Middle Aged
proteomic analysis
medicine.anatomical_structure
030220 oncology & carcinogenesis
Mesothelin
Female
Research Article
Pleural Neoplasms
GPI-Linked Proteins
Malignant pleural mesothelioma
biomarkers
proteomic analysis
secretome
proteome
cell lines
03 medical and health sciences
Cell Line
Tumor
Genetics
medicine
Biomarkers
Tumor
Humans
Molecular Biology
Settore BIO/10 - BIOCHIMICA
Aged
Prosaposin
Pleural mesothelioma
business.industry
Mesothelioma
Malignant
biomarkers
medicine.disease
secretome
ROC Curve
Cell culture
Case-Control Studies
Cancer research
biology.protein
business
Zdroj: Cancer Genomics Proteomics
Popis: Background Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome. Materials and methods The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects. Results Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy. Conclusion A panel of the putative biomarkers represents a promising tool for MPM diagnosis.
Databáze: OpenAIRE
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