TheDrosophilaLkb1 kinase is required for spindle formation and asymmetric neuroblast division
| Autor: | Maria Grazia Giansanti, Violaine Mottier, Silvia Bonaccorsi, Byron C. Williams, Bonnie J. Bolkan, Michael L. Goldberg, Maurizio Gatti |
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| Rok vydání: | 2007 |
| Předmět: |
neuroblasts
Immunoblotting Peutz-Jeghers Syndrome Spindle Apparatus Biology medicine.disease_cause Chromosome segregation AMP-Activated Protein Kinase Kinases lkb1 Neuroblast medicine Animals Drosophila Proteins Molecular Biology spindle formation Cytokinesis Neurons Genetics MELANOGASTER Mutation Brain drosophila asymmetric division Cell biology Spindle apparatus ELL-CYCLE PROGRESSION PINS Stem cell division Microscopy Fluorescence Protein Kinases Multipolar spindles POLARITY Developmental Biology Asymmetric neuroblast division |
| Zdroj: | Development (Camb.) 134 (2007): 2183–2193. doi:10.1242/dev.02848 info:cnr-pdr/source/autori:Bonaccorsi S, Mottier V, Giansanti MG, Bolkan BJ, Williams B, Goldberg ML, Gatti M./titolo:The Drosophila Lkb1 kinase is required for spindle formation and asymmetric neuroblast division./doi:10.1242%2Fdev.02848/rivista:Development (Camb.)/anno:2007/pagina_da:2183/pagina_a:2193/intervallo_pagine:2183–2193/volume:134 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | We have isolated lethal mutations in the Drosophila lkb1 gene(dlkb1), the homolog of C. elegans par-4 and human LKB1 (STK11), which is mutated in Peutz-Jeghers syndrome. We show that these mutations disrupt spindle formation, resulting in frequent polyploid cells in larval brains. In addition, dlkb1 mutations affect asymmetric division of larval neuroblasts (NBs); they suppress unequal cytokinesis, abrogate proper localization of Bazooka, Par-6, DaPKC and Miranda, but affect neither Pins/Gαi localization nor spindle rotation. Most aspects of the dlkb1 phenotype are exacerbated in dlkb1 pins double mutants, which exhibit more severe defects than those observed in either single mutant. This suggests that Dlkb1 and Pins act in partially redundant pathways to control the asymmetry of NB divisions. Our results also indicate that Dlkb1 and Pins function in parallel pathways controlling the stability of spindle microtubules. The finding that Dlkb1 mediates both the geometry of stem cell division and chromosome segregation provides novel insight into the mechanisms underlying tumor formation in Peutz-Jeghers patients. |
| Databáze: | OpenAIRE |
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