Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects
| Autor: | Ui Won Jung, Seong-Ho Choi, Kyoo-Sung Cho, Jee Hee Jung, Yoo Jung Um, Chang-Sung Kim, Jeong-Ho Yun |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Bone Regeneration Time Factors Bone Matrix Bone Morphogenetic Protein 2 Biocompatible Materials medicine.disease_cause Osteocytes Bone morphogenetic protein 2 Rats Sprague-Dawley Calcification Physiologic Bone Marrow Osteogenesis Transforming Growth Factor beta Internal medicine Absorbable Implants Escherichia coli Image Processing Computer-Assisted medicine Animals Bone formation Bone regeneration General Dentistry Drug Carriers Osteoblasts Calvarial defect Chemistry Skull Bone area medicine.disease Recombinant Proteins Rats Surgery Disease Models Animal Endocrinology Otorhinolaryngology Connective Tissue Bone Morphogenetic Proteins Collagen Block type Oral Surgery Calcification |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 111:298-305 |
| ISSN: | 1079-2104 |
| DOI: | 10.1016/j.tripleo.2010.05.011 |
| Popis: | Objectives The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model. Study design Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals. Results On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio ( P Conclusion ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2. |
| Databáze: | OpenAIRE |
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