Identification of glomerular immune deposits in cryoglobulinemia glomerulonephritis
| Autor: | Aldo Castiglione, Ettore Sabadini, Giuseppe D'Amico, Renato Alberto Sinico, Christopher G. Winearls, A Fornasieri |
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| Přispěvatelé: | Sinico, R, Winearls, C, Sabadini, E, Fornasieri, A, Castiglione, A, D'Amico, G |
| Rok vydání: | 1988 |
| Předmět: |
Idiotype
Pathology medicine.medical_specialty medicine.drug_class Renal glomerulus Biopsy Immunoglobulin Idiotype Kidney Glomerulus Immunoenzyme Technique Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody Immunoenzyme Techniques Mice Glomerulonephritis Immunoglobulin Idiotypes Immunopathology medicine Animals Humans Glomerulonephriti medicine.diagnostic_test Animal Antibodies Monoclonal medicine.disease Cryoglobulinemia Immune complex Nephrology Immunology Renal biopsy Kidney Glomerulu Human |
| Zdroj: | Kidney international. 34(1) |
| ISSN: | 0085-2538 |
| Popis: | Identification of glomerular immune deposits in cryoglobulinemia glomerulonephritis. To provide further evidence of the nature of intraglomerular immune deposits in essential mixed cryoglobulinemia (EMC), we used two mouse monoclonal antibodies against cross-reactive idiotypes present on monoclonal rheumatoid factors (MoRFs) from patients with type II-EMC. MoAb Cc1 reacted with 9 of 16 circulating IgMk MoRFs tested, and MOAb Lc1 with four of the remaining. Using indirect immunofluorescence and immunoperoxidase techniques, we could identify the same cross-reactive idiotype of the serum MoRF in the renal biopsy specimens from 11 of 13 patients with EMC glomerulonephritis. Kidney specimens from the three patients, whose MoRF was not recognized by MoAbs Cc1 and Lc1, were negative. Two out of 30 control renal biopsies from patients with other forms of glomerulonephritis were shown to contain idiotype (Cc1 and Lc1) positive material. Both patients had serum polyclonal RF which could account for this finding. In conclusion, our results provide direct evidence that serum cryo-MoRF participate in the formation of glomerular immune deposits and, presumably, in the pathogenesis of renal damage in EMC glomerulonephritis. |
| Databáze: | OpenAIRE |
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