An in vitro method to keep human aortic tissue sections functionally and structurally intact
Autor: | Gerard Pals, Willem Wisselink, René J. P. Musters, Arjan W.J. Hoksbergen, J.K. Kievit, Behrouz Zandieh-Doulabi, Dimitra Micha, Niels Keekstra, Menno E. Groeneveld, Arno M. Wiersema, Natalija Bogunovic, Jan D. Blankensteijn, Kak K. Yeung, Jorn P. Meekel, Hans W.M. Niessen |
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Přispěvatelé: | Surgery, Physiology, ACS - Atherosclerosis & ischemic syndromes, Human genetics, Amsterdam Movement Sciences - Restoration and Development, Cardio-thoracic surgery, Pathology, ACS - Heart failure & arrhythmias, AGEM - Digestive immunity, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, ACS - Microcirculation, VUmc - School of Medical Sciences, Oral Cell Biology |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Pathology
medicine.medical_specialty Cell type Science 030204 cardiovascular system & hematology Biology Immunofluorescence Article 03 medical and health sciences 0302 clinical medicine Smooth muscle medicine Aortic tissue Humans Aorta Tissue Survival Multidisciplinary medicine.diagnostic_test Pathophysiology In vitro Aortic Aneurysm Gene Expression Regulation 030220 oncology & carcinogenesis Medicine Immunostaining Ex vivo |
Zdroj: | Scientific Reports, 8(1):8094. Nature Publishing Group Meekel, J P, Groeneveld, M E, Bogunovic, N, Keekstra, N, Musters, R J P, Zandieh-Doulabi, B, Pals, G, Micha, D, Niessen, H W M, Wiersema, A M, Kievit, J K, Hoksbergen, A W J, Wisselink, W, Blankensteijn, J D & Yeung, K K 2018, ' An in vitro method to keep human aortic tissue sections functionally and structurally intact ', Scientific Reports, vol. 8, no. 1, 8094 . https://doi.org/10.1038/s41598-018-26549-4 Scientific Reports Scientific reports, 8(1):8094. Nature Publishing Group Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
ISSN: | 2045-2322 |
Popis: | The pathophysiology of aortic aneurysms (AA) is far from being understood. One reason for this lack of understanding is basic research being constrained to fixated cells or isolated cell cultures, by which cell-to-cell and cell-to-matrix communications are missed. We present a new, in vitro method for extended preservation of aortic wall sections to study pathophysiological processes. Intraoperatively harvested, live aortic specimens were cut into 150 μm sections and cultured. Viability was quantified up to 92 days using immunofluorescence. Cell types were characterized using immunostaining. After 14 days, individual cells of enzymatically digested tissues were examined for cell type and viability. Analysis of AA sections (N = 8) showed a viability of 40% at 7 days and smooth muscle cells, leukocytes, and macrophages were observed. Protocol optimization (N = 4) showed higher stable viability at day 62 and proliferation of new cells at day 92. Digested tissues showed different cell types and a viability up to 75% at day 14. Aortic tissue viability can be preserved until at least 62 days after harvesting. Cultured tissues can be digested into viable single cells for additional techniques. Present protocol provides an appropriate ex vivo setting to discover and study pathways and mechanisms in cultured human aneurysmal aortic tissue. |
Databáze: | OpenAIRE |
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