Evaluating a New High-throughput Twin-Arginine Translocase Assay in Bacteria for Therapeutic Applications
| Autor: | Shridhar Chougule, Sureshkumar Ramasamy, Deepanjan Ghosh, Vellore Sunder Avinash |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Arginine 030106 microbiology Virulence Applied Microbiology and Biotechnology Microbiology Colanic acid 03 medical and health sciences Polysaccharides Polysaccharide biosynthesis Translocase Glucans Enzyme Assays Twin-Arginine-Translocation System chemistry.chemical_classification Bacteria biology Membrane Transport Proteins General Medicine biology.organism_classification High-Throughput Screening Assays Protein Transport 030104 developmental biology Enzyme Biochemistry chemistry biology.protein Function (biology) |
| Zdroj: | Current Microbiology. 74:1332-1336 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | The twin-arginine translocase (Tat) pathway is involved in the transport of folded proteins in bacteria, and has been implicated in virulence and pathogenesis. A simple but efficient assay based on the quantification of the exopolysaccharide colanic acid was developed as a new means to study Tat function. Colanic acid contains a methylpentose (L-fucose) component, and its production is directly linked to the Tat pathway through the transport of enzymes involved in polysaccharide biosynthesis. Monitoring of L-fucose levels can be applied for identification of new Tat substrates and high-throughput screening of Tat inhibitors for therapeutic applications. |
| Databáze: | OpenAIRE |
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