Fast determination of basic drugs in plasma by gas chromatography with nitrogen phosphorus detector

Autor:	Luana Letícia da Silva, Rafael Lanaro, Bárbara Spaniol, Marina Venzon Antunes, Lilian de Lima Feltraco Lizot, Rafael Linden
Jazyk:	portugalština
Rok vydání:	2012
Předmět:	lcsh:Chemistry chemistry.chemical_compound Chromatography lcsh:QD1-999 Chemistry Stationary phase Human plasma General Chemistry drug screening Butyl acetate emergency toxicology GC-NPD
Zdroj:	Química Nova, Volume: 35, Issue: 6, Pages: 1222-1227, Published: 2012 Química Nova v.35 n.6 2012 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Vol 35, Iss 6, Pp 1222-1227 (2012)
Popis:	A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.
Databáze:	OpenAIRE
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