Disposition of Cannabinoids in Oral Fluid after Controlled Around-the-Clock Oral THC Administration
| Autor: | Marilyn A. Huestis, William D. Darwin, Garry Milman, David A. Gorelick, Deanna L. Kelly, Allan J. Barnes, David M. Schwope, Eugene W. Schwilke, Robert S. Goodwin |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Marijuana Abuse Time Factors Adolescent medicine.medical_treatment Clinical Biochemistry Pharmacology Article Matrix (chemical analysis) Young Adult chemistry.chemical_compound Oral administration medicine Humans Dronabinol Saliva biology Biochemistry (medical) biology.organism_classification Substance Abuse Detection chemistry Cannabinol Cannabinoid Cannabis Sample collection Cannabidiol medicine.drug |
| Zdroj: | Clinical Chemistry. 56:1261-1269 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/clinchem.2009.141853 |
| Popis: | BACKGROUND Oral fluid, a promising alternative matrix for drug monitoring in clinical and forensic investigations, offers noninvasive sample collection under direct observation. Cannabinoid distribution into oral fluid is complex and incompletely characterized due to the lack of controlled drug administration studies. METHODS To characterize cannabinoid disposition in oral fluid, we administered around-the-clock oral Δ9-tetrahydrocannabinol (THC) (Marinol®) doses to 10 participants with current daily cannabis use. We obtained oral fluid samples (n=440) by use of Quantisal™ collection devices before, during, and after 37 20-mg THC doses over 9 days. Samples were extracted with multiple elution solvents from a single SPE column and analyzed by 2-dimensional GC-MS with electron-impact ionization for THC, 11-hydroxy-THC (11-OH-THC), cannabidiol, and cannabinol and negative chemical ionization for 11-nor-9-carboxy-THC (THCCOOH). Linear ranges were 0.5–50 μg/L, with the exception of cannabinol (1–50 μg/L) and THCCOOH (7.5–500 ng/L). RESULTS THCCOOH was the most prevalent analyte in 432 samples (98.2%), with concentrations up to 1117.9 ng/L. In contrast, 11-OH-THC was not identified in any sample; cannabidiol and cannabinol were quantified in 3 and 8 samples, respectively, with maximum concentrations of 2.1 and 13 μg/L. THC was present in only 20.7% of samples, with highest concentrations near admission (median 4.2 μg/L, range 0.6–481.9) from previously self-administered smoked cannabis. CONCLUSIONS Measurement of THCCOOH in OF not only identifies cannabis exposure, but also minimizes the possibility of passive inhalation. THCCOOH may be a better analyte for detection of cannabis use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|