Control of histone gene expression in Physarum polycephalum : I protein synthesis during the cell cycle

Autor:	P N, Schofield, I O, Walker
Rok vydání:	1982
Předmět:	Histones Physarum Gene Expression Regulation Cell Cycle Electrophoresis Polyacrylamide Gel Cell Biology Interphase
Zdroj:	Journal of Cell Science. 57:139-150
ISSN:	1477-9137 0021-9533
DOI:	10.1242/jcs.57.1.139
Popis:	Synchronous cultures of Physarum polycephalum were pulsed with [3H] lysine hydrochloride in S and G2 phases of the cell cycle. Plasmodial extracts were separated into nuclear, ribosomal and acid-soluble post-ribosomal cytoplasmic fractions. Core histones could be detected by staining in the nuclear fractions of both S and G2 phases, but were not detected by staining in the cytoplasmic fractions. Newly synthesized histone was present in S-phase nuclei but not in S-phase cytoplasm. The specific activity of newly synthesized histone in G2-phase nuclei decreased by at least 95% compared to S phase and no newly synthesized histone was observed in G2-phase cytoplasmic fractions. Thus histone synthesis is restricted to 5 phase. There are no free pools of histone in the cytoplasm of Physarum in either S or G2 phases of the cell cycle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7beb18b7b834e39426e6fa21483125f6 https://doi.org/10.1242/jcs.57.1.139 Zobrazit plný text záznamu